Determination of the Contents of Seven Active Components in FRUCTUS EVODIAE

摘要： [Objective] To establish a HPLC/DAD (photodiode-array detection) method for simultaneous determination of synephrine, chlorogenic acid, rutin, hyperoside, quercetin, evodiamine, and rutaecarpine in FRUCTUS EVODIAE. [Method] The samples were prepared with 50% methanol aqueous solution. The chromatographic separation was carried out on a C18 column (250 mm×4.6 mm, 5 μm) and the acetonitrile-1% acetic acid was mobile phase with gradient dilution. The flow rate was 1.0 ml/min, and the detection wavelength was 254 nm for rutin, hyperoside and quercetin, 270 nm for synephrine and rutaecarpine, and 340 nm for chlorogenic acid and evodiamine, respectively. [Result] The separation of synephrine, chlorogenic acid, rutin, hyperoside, quercetin, evodiamine, and rutaecarpine was relatively ideal.Their average recovery rates approached 100%, and RSD values were all below 3.1%. [Conclusion] The method was suitable for the simultaneous determination of synephrine, chlorogenic acid, rutin, hyperoside, quercetin, evodiamine and rutaecarpine, and it provided better technical support for the quality evaluation of FRUCTUS EVODIAE. ... （共4页）