IdentificationofLablabSemenAlbumbyDNABarcodeTechnology
摘要: [Objectives]ToidentifyITS2barcodeofLablabSemenAlbumanditsadulterants,andprovideanewmethodfortheidentificationofLablabSemenAlbum.[Methods]TheITS2sequencewasamplifiedbyPCRandsequencedbi-directionally.AftersplicingbyCodonCodeAligner,thedatawereprocessedwiththeaidMEGAsoftwaretoconstructtheclusterdendrogram(neighbor-joining,NJtree).[Results]TheITS2sequenceofLablabSemenAlbumhadlengthof218bp;theconstructedclusterdendrogramindicatedthatallspeciesweremonophyleticandcouldbedistinguishedfromotherspecies.[Conclusions]TheITS2barcodecanbeusedforrapididentificationofLablabSemenAlbumanditsadulterantsandthisexperimentfurtherverifiedthatDNAbarcodetechnologyiseffectiveinidentificationoftraditionalChinesemedicines. ...
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