Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells

摘要： <正> Objective: To evaluate whether realgar could down-regulate human telomerase reverse transcriptase(hTERT) gene expression and telomerase activity in acute promyelocytic leukemia cell line-NB4 cells. Methods: Theexpression of hTERT-mRNA was analyzed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay (PCR-ELISA).Flow cytometry using PI staining was applied to analyze the cell cycle and apoptosis. Results: Treatment of NB4 cellswith 155, 300, 600 μg/L realgar reduced telomerase activity significantly accompanying with decrease of hTERT-mR-NA and increasing cell apoptosis. G2/M phase arrest appeared when treated with realgar in 300, 600 μg/L. Condu-sion: It is suggested that telomerase activity of NB4 cells can be specifically inhibited by realgar through the down-regu-lation of hTERT gene expression. G_2/M phase arrest and apoptosis by realgar in NB4 cells might be related to the re-ductio ... （共5页）