Protoplast fusion is an effective method for direct intergeneric genetransfer into plant protoplasts as well as for creating new sources of cytoplasmic variability,transfer and recombining organelle genomes,and provides a suitable technique for studying c...[继续阅读]

    Parasexual hybridization is a process to develop hybrid plants by fusion of protoplasts from different species or related plants differing in theircytoplasms. In a broad sense,it also may include inserting organellessuch as nuclei,plastids,mitochondria,an...[继续阅读]

    Tobacco has been on the forefront of plants modified by genetic engineering,being the first plant used to express an engineered foreign gene.These first transformations of tobacco involved the use of Agrobacteriumtumefaciens as a vector. Since that time,s...[继续阅读]

    The advent of molecular techniques has recently provided novelstrategies for control of plant virus disease which make use of“pathogenderived resistance”. Pathogen-derived structural or non-structural genesequences expressed in the host plant in excess am...[继续阅读]

    The engineering of insect resistance was first demonstrated in tobaccoby the expression of insecticidal crystal proteins (ICPs) derived from B.thuringiensis (Bt),a Gram-positive,spore-forming soil bacterium.The lepidopteran-specific CrylA (b) crystal prot...[继续阅读]

    Numerous studies involving tobacco as a “biofactory” have been initiated where this solanaceous plant has been used as the recipient of genesthat encode protein products of commercial value.In work reported by Hiatt et al. ,the γ-and κ-chain cDNAs wereclo...[继续阅读]

    Biotechnology,of which recombinant DNA technology is an important sub-discipline,has a long tradition in the production of food and flavors. Around 3500 B. C.,humans first started to use microbes for theproduction of wine,beer,bread and many other foods t...[继续阅读]

    AbstractA convergence of findings indicates that smoking can relieve dysphoric mood and help individuals perform more effectively and cope withstress. For many individuals these beneficial effects of smoking appear tobe their primary reason for smoking. M...[继续阅读]

    By Gio Batta GoriThe Health Policy Center,Bethesda,Maryland,USAAbstractThe grounds for official policy claims that environmental tobaccosmoke is a major health risk remain speculative. In scientific terms,available reports suggest either a slight increase...[继续阅读]

    Donald F. DurocherAbstractOver the past fifty years,sweeping technological changes have resulted in inexpensive tobacco products manufactured to exacting tolerancesusing state - of - the - art technologies. The TCRC has played a key rolein reporting and d...[继续阅读]