转化生长因子TGF-β在不同时期血管瘤中的表达与肥大细胞的相关性

摘要： 目的:探讨转化生长因子TGF-β在血管瘤自然消退过程中的表达变化及其与肥大细胞的相关性。方法:收集血管瘤标本56例,按发病年龄,结合血管瘤组织学特征,将其分为增生早期、中期、晚期和退化早期、中期、晚期。分别采用甲苯胺蓝染色和免疫组织化学SABC法,检测肥大细胞数量和转化生长因子TGF-β的表达;采用显微图像分析软件测定TGF-β在不同时期血管瘤中免疫组织化学染色的平均阳性面积率,同时测定各期肥大细胞的平均计数值;采用SPSS11.0软件包对结果进行单因素方差分析及Pearson相关分析。结果:随着血管瘤的自然演变过程,肥大细胞数目从增生早期至增生晚期逐渐增多,从退化早期至退化晚期又逐渐减少;增生晚期肥大细胞计数与增生早、中期相比有显著差异(P<0.01),退化早期肥大细胞计数与退化中、晚期相比有显著差异(P<0.01);TGF-β从增生早期至增生晚期的表达逐渐增强,从退化早期至退化晚期的表达又逐渐减弱,并且在增生晚期的表达与增生早、中期相比有显著差异(P<0.01),在退化早期的表达与退化中、晚期相比有显著差异(P<0.01);不同时期血管瘤中TGF-β表达的平均阳性面积率与肥大细胞数目呈显著正相关(P<0.01)。结论:肥大细胞随着血管瘤的自然消退,其数量呈规律性变化,肥大细胞可能对血管瘤的消退进程起促进作用;TGF-β可能具有促进血管内皮细胞凋亡,从而发挥促血管瘤消退的作用;肥大细胞可能通过分泌TGF-β而发挥促血管瘤自然消退的作用。
PURPOSE: To investigate the expression of TGF-β and its relationship with mast cell in the different stages of vessel differentiation in human dermal hemangiomas. METHODS: 56 cases of human dermal hemangioma specimen were collected. The evolutional process of hemangioma were divided into six stages, which were the early stage, intermediate stage and advanced stage of proliferating hemangioma and involuting hemangioma. Immunohistochemical staining technique(SABC) and toluidine blue (TB) staining were used to detect the expression of TGF-β and the number of mast cells in different stages of human dermal hemangioma; Micro-image analysis software was used to determine the mean value of mast cells, average positive area ratio of TGF-β in different stages of human dermal hemangioma. SPSS for Windows 11.0 software package was used to analyze the results by means of one -way ANNOVA,Student’s t test and Pearson correlation analysis. RESULTS: The number of mast cells increased gradually from the early stage of proliferating hemangioma to the advanced stage of proliferating hemangioma and decreased gradually from the early stage of involuting hemangioma to the advanced stage of involuting hemangioma. There was significant difference between the advanced stage of proliferating hemangioma and other stages of proliferating hemangioma in the number of mast cells (P<0.01). There was also significant statistical difference between the early stage of involuting hemangioma and the other stages of involuting hemangioma in the number of mast cells (P<0.01). The expression of TGF-β increased gradually from the early stage ofproliferating hemangioma to the advanced stage of proliferating hemangioma and decreased gradually from the early stage of involuting hemangioma to the advanced stage of involuting hemangioma. The expression of TGF-β in endothelial cells during the early stage of involuting hemangioma was significantly higher than the other stages in involuting hemangioma (P <0.01). The expression of TGF -β in endothelial cells during the advanced stage of proliferating hemangioma was significantly higher than the other stages in proliferating hemangioma (P <0.01). The expression level of TGF -β in endothelial cells was positively correlated with the number of mast cells in different stages of human dermal hemangioma (P<0.01). CONCLUSIONS: With the natural evolutional process of hemangioma, the number of mast cells displays regular variation and mast cell may play a promotive role of apoptosis during the spontaneous involution of human dermal hemangioma; TGF -β may promote the apoptosis of vascular endothelial cells in hemangioma and furthermore the involutional process of hemangioma; Mast cells may produce TGF-β and promote the spontaneous involution of human dermal hemangioma. ...